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This study was conducted to investigate the mechanism of microtuberization based on the potato (Solanum tuberosum L. cv. Dejima) axillary bud culture. Microtuber formation efficiency was in that order of MS with CCC 250 ㎎·L^(-1), MS with ABA 0.01 ㎎·L^(-1) and MS control, whereas microtuber were not formed in the MS medium added with GA₃ 5 ㎎·L^(-1). IEF. SDS-PAGE of proteins extracted from shoots grown under light or dark conditions of in vitro plantlets showed that protein patterns of shoot were changed greatly with molecular weights of 25-to 80-kDa in size. Changes of proteins in vitro plantlets cultured far 1 week under the treatment of various growth regulators showed that protein patterns differed with molecular weights of 24- to 65-kDa. In immunoblottings, the 22-kDa KPPI specific antibody was cross-reacted with proteins from shoot treated with CCC 250 ㎎·L^(-1), ABA 0.01 ㎎·L^(-1), and control, but not reacted with shoot treated with GA₃ 5 mg-L-~ for 1~4 weeks of in vitro plantlets. The proteins extrated from in vitro potato plantlets cultured for 1 week, patatin antibody was reacted with proteins from all shoots, regardless of the treatment of growth regulators.
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